Effect of Bifidobacterium on the expression of β-defensin-2 in intestinal tissue of neonatal rats with necrotizing enterocolitis / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To study the effect of Bifidobacterium on the expression of β-defensin-2 (BD-2) in intestinal tissue of neonatal rats with necrotizing enterocolitis (NEC).</p><p><b>METHODS</b>A total of 40 rats were randomly divided into four groups: normal control, Bifidobacterium control, NEC model, and Bifidobacterium treatment, with 10 rats in each group. A rat model of NEC was induced by hypoxia, cold stimulation, and artificial feeding. The rats in the Bifidobacterium control and Bifidobacterium treatment groups were given Bifidobacterium via the gastric tube after cold stimulation once a day for three consecutive days. The morphological changes of the terminal ileum were observed under a light microscope and the intestinal injury score was determined. Immunohistochemistry and qRT-PCR were used to measure the protein and mRNA expression of BD-2 in the ileal mucosal tissue.</p><p><b>RESULTS</b>The NEC model group had a significantly higher intestinal injury score than the normal control, Bifidobacterium control, and Bifidobacterium treatment groups (P<0.05). The Bifidobacterium treatment group had a significantly higher intestinal injury score than the normal control and Bifidobacterium control groups (P<0.05). The mRNA and protein expression of BD-2 in the normal control group was significantly lower than in the Bifidobacterium control, NEC model, and Bifidobacterium treatment groups (P<0.05). The Bifidobacterium control group had significantly higher mRNA and protein expression of BD-2 than the NEC model and Bifidobacterium treatment groups (P<0.05). The Bifidobacterium treatment group had significantly higher mRNA and protein expression of BD-2 than the NEC model group (P<0.05).</p><p><b>CONCLUSIONS</b>Bifidobacterium can induce the expression of BD-2 in intestinal tissue of rats and reduce inflammatory response by increasing the expression of BD-2. This provides a protective effect on neonatal rats with NEC.</p>

Association between surfactant protein B gene polymorphisms and susceptibility to bronchopulmonary dysplasia in preterm infant / 中华实用儿科临床杂志

Objective To investigate the distribution of surfactant protein B (SP-B) gene single nucleotide polymorphisms and to study the association between the SP-B gene polymorphisms and bronchopulmonary dysplasia (BPD) in preterm infant.Methods Forty-two preterm infants with BPD and 68 preterm infants without BPD (control group) were selected.Polymerase chain reaction with restriction fragment length polymorphism was used to establish the genotype and allele frequencies of the SP-B-18A/C,SP-B 1580C/T,SP-B 8714G/C single nucleotide the polymorphisms,and then the association between the polymorphisms and BPD was analyzed.Results The frequencies of genotype CC,AC,AA of SP-B-18A/C in BPD group and control group were 35.7％,52.4％,11.9％ and 32.3％,47.7％,20.0％,respectively.The frequencies of genotype CC,GC,GG of SP-B 8714G/C in BPD group and the control group were 26.2％,54.8％,19.0％ and 27.7％,58.5％,13.8％,respectively.The frequencies of genotype CC,CT,TT of SP-B 1580C/T in BPD group and control group were 66.7 ％,26.2％,7.1％ and 40.0％,47.7 ％,12.3 ％,respectively.The frequencies of allele C and allele A of SP-B-18A/C in BPD group and control group were 61.9％,38.1％ and 56.2％,43.8％,respectively.The frequencies of allele G and allele C of SP-B 8714G/C in BPD group and control group were 53.6％,46.8％ and 56.9％,43.1％,respectively.The frequencies of allele C and allele T of SP-B 1580C/T in BPD group and control group were 79.8％,20.2％ and 63.8％,36.2％,respectively.SP-B-18A/C,SP-B 1580C/T,SP-B 8714G/C were found to be polymorphic.The frequencies of genotype CC of SP-B 1580C/T in BPD group were significantly higher than those in the controls (x2 =7.26,P ＜ 0.05).The frequencies of allele C of SP-B 1580C/T were also statistically different between BPD group and control group(x2 =6.17,P ＜0.05).The C allele increased the risk of BPD(OR =2.23,95％ CI:1.18-4.24).There was no significant difference between the 2 groups in the frequencies of genotype and allele of SP-B-18A/C and SP-B 8714G/C.Conclusions SP-B 1580C/T polymorphism has association with the etiology of BPD and may serve as the susceptibility gene for BPD,the C allele increase the risk of BPD.SP-B-18A/C and SP-B 8714G/C do not have association with the etiology of BPD.

Relationship between pulmonary surfactant-associated protein B polymorphisms and the susceptibility to neonatal respiratory distress syndrome / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To study the relationship between pulmonary surfactant-associated protein B (SP-B) gene polymorphisms and their susceptibility to neonatal respiratory distress syndrome (RDS).</p><p><b>METHODS</b>Eighty-eight preterm infants with RDS (RDS group) and 103 infants without RDS (control group) were enrolled. The genomic DNA was isolated using DNA kits. Polymerase chain reaction with restriction fragment length polymorphism technique was used to detect the genotype and allele frequency of the SP-B -18A/C and SP-B 1580C/T single nucleotide polymorphisms. The association between the polymorphisms and RDS was analyzed.</p><p><b>RESULTS</b>SP-B -18A/C and SP-B 1580C/T were found to be polymorphic in both RDS and control groups. The frequencies of CC genotype (X2=12.26, P<0.01) and C allele (X2=11.97, P<0.01) of SP-B 1580C/T were significantly higher in the RDS group than in the control group. The C allele significantly increased the risk of RDS (OR=2.26, 95%CI: 1.42-3.60). The frequencies of genotype and allele of SP-B -18A/C showed no significant difference between the two groups.</p><p><b>CONCLUSIONS</b>SP-B 1580C/T polymorphism contributes to the etiology of RDS and may serve as the susceptibility gene for RDS. The C allele increases the risk of RDS. SP-B -18A/C shows no association with the etiology of RDS.</p>

A meta-analytic review of ERCC1/MDR1 polymorphism and chemosensitivity to platinum in patients with advanced non-small cell lung cancer / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Platinum-based regimens are used as standard first-line chemotherapy in non-small cell lung cancer (NSCLC) patients. To study if pharmacogenetic approach may allow a tailored selection of platinum chemotherapy for advanced NSCLC, we performed a meta-analysis to compare chemosensitivity to platinum with different ERCC1 C118T/ MDR1 C3435T single-nucleotide polymorphism (SNP).</p><p><b>METHODS</b>Relevant studies were identified by searching the PubMed, Embase, Cochrane, OVID, Springer, EBSCO and CNKI databases. Inclusion criteria were patients with advanced NSCLC who received platinum-based chemotherapy, an evaluated polymorphism of ERCC/MDR1 and overall response rate. We excluded duplicate publications, letters and review articles. The RevMan 4.2 and STATA 11 package were used to do comprehensive quantitative assessment.</p><p><b>RESULTS</b>A total of 11 studies were included in this meta-analysis. For studies evaluating ERCC1 C118T, test for heterogeneity was done (χ(2) = 13.41, P = 0.1), and the odds ratio (OR) for the wild-type C/C genotype versus the heterozygous C/T and T/T genotypes was 1.50 (95% CI 1.09 - 2.06, P = 0.01). In four studies evaluating MDR1 polymorphism, test for heterogeneity was also done (χ(2) = 3.22, P = 0.36), and the OR for the wild-type C/C genotype versus the heterozygous C/T and T/T genotypes was 2.30 (95% CI 1.44 - 3.68, P = 0.0005).</p><p><b>CONCLUSIONS</b>The results indicated that platinum-based chemotherapy sensitivity was significantly associated with polymorphism of ERCC1 C118T and MDR1 C3435T SNP. In further perspective studies, the ERCC1/MDR1 SNPs might serve as simple and less invasive biomarkers for personalized chemotherapy with platinum-based anticancer drugs.</p>